Nikon N-Storm Super Resolution Microscope Information and Specifications

Nikon N-Storm Super Resolution Microscope

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Nikon Storm Microscope and Slide Image

Instrument Description

Instrument Features

The Nikon "Storm" system is an N-STORM super resolution microscope,capable of achieving 2 0 m XY and 50mm Z resolution - 10X increase inresolution over a traditional fluorescence microscope. STochastic Optical Reconstruction Microscopy (STORM) is our most advanced imaging modality offered at the MSR and is ideal for experimental questions requiring nanoscale resolution. This instrument is capable of acquiring up to 3 fluorescence channels and has the optics to acquire 3D STORM images. Due to the advanced nature of STORM imaging, the MSR offers an Assisted STORM Imaging Service to assist users with no background in STORM. This service includes the preparation of MSR supplied STORM buffers and MSR staff performing the STORM imaging with user guidance.
 

  • Super resolution imaging 
    • ( 2 0 m XY and 5 0 m Z resolution)
  • Optics to acquire up to 3 fluorescence channels
    • (488nm / 561nm / 640nm)
  • 3D STORM imaging 
    • with astigmatism lens
  • Photoactivated Localization Microscopy (PALM) imaging for samples with photoconvertible fluorescent proteins

     


Instrument Details
 
Available Lasers

405m / 488m / 561nm at 100mW 

640 nm at 125mW

Available Objective Lenses
  • 20x Plan Apo (NA = 0.75; WD = WDmm; DIC)
  • 100x Oil Plan Apo (NA = 1.49; WD = 120um; DIC)
Available Camera

Andor iXon Ultra 897

  • Back-illuminated EMCCD sensor
  • Pixel size = 16um
  • Sensor size= 512x512
Emission Filters
  • DAPI - 4 5 5 m / 50nm 
  • GFP - 5 2 5 m / 36nm 
  • Cy3 - 605nm / 52nm 
  • Cy5 - 705nm / 72nm
Supported Samples

IMPORTANT: Samples must in the format below:

  • Glass-bottom Dishes (35mm) with No. 1.5 coverslips Recommended No. 1.5H coverslips
    Mattek P35G-0.170-14-C
  • Unmounted samples only (store in 1x PBS)