Nikon Crest Spinning-Disk Confocal Microscope
Instrument Details
Available Lasers
405nm / 445nm / 477nm / 520nm / 545nm / 635m / 730nm
Available Objective Lenses
- 4x Plan Apo (NA = 0.13; WD = 16.5mm; Phase)
- 10x Plan Fluor (NA = 0.30; WD = 15.2mm; Phase)
- 20x Plan Apo (NA = 0.75; WD = 1mm; DIC)
- 40x Plan Apo (NA = 0.95; WD = 250um; DIC)
- 60x Oil Plan Apo (NA = 1.40; WD = 130um; DIC)
Available Detectors
- Photometrics Prime 95B (fluorescence)
- Back-illuminated sCMOS sensor with 95% QE
- Pixel size = 11um
- Sensor Size = 1608 × 1608
- Nikon DS-Fi3 (color)
- Color CMOS sensor
- Pixel Size = 2.4um
- Sensor Size 2880 x 2048
Emission Filters
- DAPI - 438nm / 24nm
- CFP - 485nm / 20nm
- GFP - 511nm / 20nm
- YFP - 560nm / 25nm
- Cy3 - 595nm / 31nm
- Cy5 - 685nm / 40nm
- Cy7 - 819nm /44nm
Supported Samples
- Slides
- (1x3 and 2x3; No. 1.5 coverslips)
- Glass-bottom Dishes
- (35mm/60mm; No. 1.5 coverslips)
- Glass-bottom Multi-well Plates (all well sizes)
- Glass-bottom Chamber Slides (all well sizes)