Nikon Crest Spinning-Disk Confocal Microscope Information and Specifications

Nikon Crest Spinning-Disk Confocal Microscope

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Nikon Crest Microscope and Slide

Instrument Description

Instrument Features

The Nikon "Crest" system is an inverted, spinning-disk confocal microscope that allows for high-throughput, confocal imaging of cell and tissue samples.
The Crest Spinning-Disk features a wide selection of laser lines (UV- IR) and is ideal for applications that require the increased resolution and contrast of
confocal imaging, with the speed of a widefield microscope. The Crest system is equipped with a stage-top environmental incubator for live cell imaging and a back-illuminated sCMOS camera, making it ideal for imaging living samples (cells, organoids, etc.) with minimal phototoxicity. The Crest features both fluorescence and color cameras, enabling users to image datasets with IHC and fluorescent samples.
 

  • Confocal imaging 
    • (Crest X-Light V2 Spinning-Disk Optics)
  • Nikon DS-Fi3 Color and Photometrics Prime 95B sCMOS cameras
  • Live cell imaging 
    • (humidity, temperature, and CO, regulation) 
  • Laser selection for wide spectral coverage 
    • (UV- IR wavelengths) 
  • CFP/YFP filter sets for FRET imaging
  • Hardware triggering for rapid multichannel acquisition

Instrument Details
 
Available Lasers

405nm / 445nm / 477nm / 520nm / 545nm / 635m / 730nm

Available Objective Lenses
  • 4x Plan Apo (NA = 0.13; WD = 16.5mm; Phase)
  • 10x Plan Fluor (NA = 0.30; WD = 15.2mm; Phase)
  • 20x Plan Apo (NA = 0.75; WD = 1mm; DIC)
  • 40x Plan Apo (NA = 0.95; WD = 250um; DIC)
  • 60x Oil Plan Apo (NA = 1.40; WD = 130um; DIC)
Available Detectors
  • Photometrics Prime 95B (fluorescence)
    • Back-illuminated sCMOS sensor with 95% QE 
    • Pixel size = 11um
    • Sensor Size = 1608 × 1608
  • Nikon DS-Fi3 (color)
    • Color CMOS sensor
    • Pixel Size = 2.4um
    • Sensor Size 2880 x 2048
Emission Filters
  • DAPI - 438nm / 24nm
  • CFP - 485nm / 20nm
  • GFP - 511nm / 20nm
  • YFP - 560nm / 25nm
  • Cy3 - 595nm / 31nm 
  • Cy5 - 685nm / 40nm
  • Cy7 - 819nm /44nm
Supported Samples
  • Slides
    • (1x3 and 2x3; No. 1.5 coverslips)
  • Glass-bottom Dishes 
    • (35mm/60mm; No. 1.5 coverslips) 
  • Glass-bottom Multi-well Plates (all well sizes) 
  • Glass-bottom Chamber Slides (all well sizes)