Nikon AX R Laser-Scanning Confocal Microscope Information Page

Nikon AX R Laser-Scanning Confocal Microscope

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Image
Nikon AXR Microscope alongside microscopic slide

Instrument Description

Instrument Features

The Nikon "AX R" system is an inverted laser-scanning confocal microscope and is our most capable instrument. The AX R has both a traditional, high-resolution Galvano scanner and a high-speed Resonant scanner with up to 2K X 2K resolution, making this instrument capable of both high-resolution and high-speed imaging. The AX R offers the largest selection of laser lines, allowing the microscope to excite up to 7 fluorophores with clear spectral separation. This instrument has the widest range of objectives lenses, including a long working distance 20x 0.7NA objective with 2.3mm of working distance and water-immersion 40x 1.25NA objective paired and automated water immersion dispenser, ideal for long-term, live cell and multi-well plate imaging.

  • High-resolution image acquisition
    • Galvano scanner (up to 8K X 8K)
  • High-speed image acquisition 
    • with Resonant scanner (up to 2K X 2K)
  • Live cell imaging 
    • humidity, temperature, and CO2 regulation
  • Advanced multichannel imaging
    • up to 7 fluorophores per sample
  • Piezo stage with 600um travel for precise, high-speed zstack imaging 
  • Automated water dispenser for automated multi-well imaging using the water-immersion 40x objective. 

Instrument Details
 
Available Lasers

405nm / 445m / 488m / 515nm / 561m /594m/ 640m /730nm

Available Objective Lenses
  • 4x Plan Apo (NA= 0.20; WD 20mm; Phase)
  • 10x Plan Apo (NA = 0.45; WD = 4mm; Phase)
  • 20x Plan Apo (NA = 0.80; WD = 800um; DIC)
  • 20x Long WD (NA = 0.70; WD = 2.3; DIC)
  • 40x Plan Apo (NA = 0.95; WD = 250um; DIC)
  • 40x W Plan Apo (NA = 1.25; WD = 200um; DIC)
  • 60x Oil Plan Apo (NA = 1.42; WD = 150um; DIC)
Available Detectors
  • Metal-Alkali (Fixed) 
  • GaAsP PMT (Adjustable) 
  • GaAsP PMT (Adjustable) 
  • GaAsP PMT (Fixed)
  • Transmitted Light Detector (TD)
Dichroic Mirrors 
+
Beam Splitter

DM 405nm / 488m / 561nm / 640nm / 730nm 
DM 405m / 445nm / 514m / 594nm / 730nm 
BS 80 / 20

Supported Samples
  • Slides 1( x 3 and 2 x 3; No. 1.5 coverslips)
  • Glass-bottom Dishes (35mm/60mm; No. 1.5 coverslips) 
  • Glass-bottom Multi-well Plates (al wel sizes) 
  • Glass-bottom Chamber Slides (all well sizes)