Analytical Chemistry

The mission of the University of Arizona Cancer Center Analytical Chemistry Shared Resource (ACSR) is to provide Cancer Center investigators with centralized resources and expertise in performing analytical chemistry assays and pharmacokinetic (PK) and pharmacodynamic (PD) data analysis and interpretation. The Analytical Chemistry Shared Resource has accumulated considerable experience in quantitative analysis of small molecules (<1,000 daltons) in biological specimens. ACSR is capable of providing integrated PK service from the study design stage through quantification of drugs levels to PK/PD data analysis.  In addition, ACSR has developed capabilities to support targeted metabolomics analysis and untargeted metabolomics profiling. 

Specifically, the ACSR has the following aims:

  • To develop and implement chromatography-mass spectrometry-based analytical chemistry methods for quantification of cancer therapeutic and preventive agents, nutrients, carcinogens, endogenous biochemicals, and imaging agents;
  • To perform quantitative and qualitative analysis of cancer therapeutic and preventive agents, nutrients, carcinogens, endogenous biochemicals, and imaging agents;
  • To provide consultation in pharmacokinetic study design and pharmacokinetic and pharmacodynamic data analysis.

ACSR has continued to build its repository of analytical assay protocols, many of which can be readily applied to new projects, and thus the ACSR is able to support a broad spectrum of research projects in a cost-effective manner and with a rapid turnaround time. By continuing to provide state-of-the-art analytical chemistry and pharmacokinetic services, the ACSR laboratory should greatly augment pre-clinical drug discovery efforts, stimulate the incorporation of pharmacokinetic endpoints in clinical trials, and facilitate biomarker analysis in cancer prevention and control research.

Build a project, get a quote, or order a service here.

Please remember to acknowledge the Cancer Center Support Grant (P30 CA023074) when publishing manuscripts or abstracts that utilized the services of the University of Arizona Cancer Center’s Shared Resources and/or were derived from CCSG pilot funds. Suggested language: "Research reported in this [publication/press release] was supported by the National Cancer Institute of the National Institutes of Health under award number P30 CA023074.

  • Consultation on study design including selection of established, validated assays from the analytical assay protocol repository and/or development of new assays
  • Development/implementation of analytical chemistry assays for quantification of small molecules (<1,000 daltons) in biological specimens
  • Quantitative analysis of small molecules in clinical and preclinical samples using chromatography and mass spectrometry-based systems or atomic absorption spectrophotometry
  • Performance of targeted metabolomics analysis and untargeted metabolomics profiling
  • Pharmacokinetic study design, quantitative analysis of drug levels, and pharmacokinetic and pharmacodynamics data analysis

Services provided by the ACSR are essential for the assessment of drug/nutrient/carcinogen exposure and disposition, and for the measurement of endogenous biochemicals as surrogate cancer-risk biomarkers and endpoint biomarkers in intervention studies. These measurements are indispensable in many established lines of research in the University of Arizona Cancer Center, including preclinical and clinical evaluation of cancer therapeutic drugs and preventive interventions, assessment of biological, environmental, and lifestyle factors associated with cancer risk and disease progression, and identification of potential targets for intervention. The services provided by the ACSR are playing an increasingly important role in the emerging field of metabolomics that is reshaping the research directions of cancer research in general.

State-of-the-art equipment and sophisticated analytical chemistry techniques are provided by the ACSR to cover a broad variety of needs. Personnel are trained experts in the operation and maintenance of the equipment. They have the expertise to develop and optimize the appropriate methods. Major equipment includes:

  • Agilent Ultivo Triple Quadrupole Mass Spectrometer with Ultra Performance Liquid Chromatography System
  • ThermoFinnigan TSQ Quantum Ultra Mass Spectrometer with High Performance Liquid Chromatography System
  • Waters Xevo G2-S QToF Mass Spectrometer with Ultra Performance Liquid Chromatography System
  • ThermoFinnigan Trace DSQ Gas Chromatography Mass Spectrometer System
  • Perkin Elmer Aanalyst 600 Atomic Absorption Spectrophotometer System
  • Thermo TSQ Quantum Ultra Mass Spectrometer (3rd Generation) High Performance Liquid Chromatography
  • Sciex 5500 QTrap Mass Spectrometer with Ultra High Performance Liquid Chromatography
  • Agilent Intuvo 9000 Gas Chromatography with Flame Ionization Detector
  • Access to Thermo Exploris 480 with Thermo Vanquish Horizon Duo Ultra High Performance Liquid Chromatograph

Analyte

Sample Type

Instrument

2-AG(2-Arachidonoylglycerol), AEA(N-arachidonoylethanolamine)

Brain, brain perfusate

HPLC-MS/MS

 4-HPR (N-(4-hydroxyphenyl)-retinamide)

Plasma; Tissue

HPLC-FLD 

5-FU (Fluorouracil)

Cells

HPLC-MS/MS

8-Hydroxy-2'-deoxyguanosine

Urine; DNA 

HPLC-MS/MS 

8-Isoprostaglandin F2 alpha 

Urine; Plasma

HPLC-MS/MS

Acetylcholine

Mouse cortex

HPLC-MS/MS

AH1S2(iron chelator)

Mouse Plasma

HPLC-MS/MS

Alpha-tocopheryloxyacetic acid

Plasma

HPLC-MS/MS

Amino Acids

Cell Culture

HPLC-MS/MS

AMP423(naphthyl derivative of 2-cyanoaziridine-1-carboxamide)

Formulation Preparation

HPLC-MS 

Aspirin

Plasma

HPLC-MS/MS

Antalarmin 

Plasma 

HPLC-MS/MS 

Arginine/Ornithine

Intestine;
Colon Tissue

HPLC-FLD 

AZ002S

Mouse Plasma

HPLC-MS/MS

AZD/Ponatinib

Plasma

HPLC-MS/MS

Bile acids

Fecal Water; Plasma;
Intestinal fluid

HPLC-MS 

Bile acids (parent bile acids and taurine and glycine conjugates)

Gastric fluid

HPLC-MS/MS 

Bixin

Plasma

HPLC-UV

Buspirone 

Plasma 

HPLC-MS/MS 

C391

Plasma

HPLC-UV-MS

Caffeine/Caffeine metabolites 

Plasma 

HPLC-UV;
HPLC-MS/MS

Carbendazim 

Plasma 

HPLC-MS/MS 

Carotenoids

Plasma; Skin 

HPLC-FLD 

Celecoxib 

Plasma 

HPLC-UV 

Ceramide

Cells; plasma;
Urine; tissue

*HPLC-MS/MS 

Ceramide-1-Phosphate

Cells; plasma;
Urine; tissue

*HPLC-MS/MS 

cGMP

Cell Extract

HPLC-MS/MS

Chlorophyll 

Colon Tissue 

HPLC-FLD

Compound L

Mouse Plasma

HPLC-MS/MS

Cortisol/6-Hydroxycortisol 

Urine

HPLC-UV 

Cortisol/Cortisone 

Saliva

HPLC-MS/MS 

Curcumin

Plasma; Urine; Skin 

HPLC-UV;
HPLC-MS/MS

Cytarabine + nucleosides

Cells

HPLC-MS/MS

Dexmedetomidine

Urine

HPLC-MS/MS 

Dextromethorphan (DM)/DM metabolites

Urine 

HPLC-FLD 

DFMO (Alpha-difluoromethylornithine)

Plasma; Skin 

HPLC-FLD

Diacylglycerides

Cells; plasma;
Urine; tissue

*HPLC-MS/MS 

Dihydrotestosterone

Cytosolic extract

HPLC-MS

DIM (diindolylmethane)

Urine

HPLC-MS/MS

Diphenidol

Cell culture media

HPLC-MS/MS

DITPA (3,5-diiodothyropropionic acid) 

Plasma 

HPLC-MS/MS

Dopamine

Mouse Cortex

HPLC-MS/MS

Doxorubicin

Tissue (mouse tumor)

UPLC-TOF-MSe

DPC0046

Plasma

HPLC-MS/MS

Endocannabinoids

Plasma

*HPLC-MS/MS

Epinephrine

Mouse cortex

HPLC-MS/MS

Estrone/Estradiol +13 metabolites

Plasma; urine

HPLC-MS/MS

Fatty acids

Plasma

HPLC-MS/MS

FICZ(6-Formylindolo(3,2-b)carbazole)

PBS

HPLC-MS

GABA (gamma-aminobutyric acid)

Mouse Cortex; Urine

HPLC-MS/MS 

gamma-Glutamylcysteine 

Cell extract 

HPLC-FLD 

Gemcitabine Triphosphate

Blood Lymphocytes;
Cell Extract

HPLC-UV

Gemcitabine 

Plasma; Tissue; Cell Extract 

HPLC-UV;
HPLC-MS/MS

Genistein

Plasma

HPLC-MS/MS

Glutamate

Mouse Cortex

HPLC-MS/MS 

Glyoxal, Methyl glyoxal

Cell extract

GC-MS

5-,8-,11-,12-,15-,20-HETEs (hydroperoxyeicosatetraenoic acid)

Lung Tissue 

HPLC-MS/MS

Hexosylceramide/LactosylCer

Cells; plasma; Urine; tissue

*HPLC-MS/MS 

HPMA(N-(2-Hydroxypropyl)methacrylamide), SPMA
(S-phenyl mercapturic acid),
HMPMA(3-hydroxy-1-methylpropylmercapturic acid)

Urine

HPLC-MS/MS

Imexon

Plasma 

HPLC-UV 

Iron

Cell Extract

AAS

Isothiocyanate-glutathione conjugates

Cultured cells 

HPLC-MS/MS 

Kynurenine Pathway Metabolites***

Urine, plasma, liver

HPLC-MS/MS

LTE4(Leukotriene E4)

Urine

HPLC-MS/MS

Limonene 

Plasma; breast tissue,
adipose tissue 

HPLC-MS/MS;
GC-MS 

Losartan/Losartan Metabolites

Urine; Plasma

HPLC-UV;
HPLC-MS/MS

Malondialdehyde

Cell extract

GC-MS

Melphalan/Melphalan analogues

Dosing Preparation 

HPLC-MS/MS

Metformin

Plasma; Cell culture media;
Tissue

HPLC-MS/MS 

Methyl-4-aminosalicylate

Pure Standard

HPLC-UV

Metronidazole

Cells

HPLC-MS/MS

Midazolam/ Midazolam Metabolites

Plasma

HPLC-MS/MS

Napthazarin

Pure Standard

HPLC-UV-MS/MS

Nitotinic acid/Nicotinamide

Plasma 

HPLC-MS/MS

Nitrite

Cell culture media

HPLC-FLD

Norepinephrine

Mouse cortex

HPLC-MS/MS

NPA-093

Plasma

HPLC-UV

Nucleoside Triphosphates/deoxyNucleoside Triphosphates

Cell extract

HPLC-MS/MS

Omeprazole/Metabolites

Plasma

HPLC-MS/MS

Oxylipins(69 individual**)

Plasma

UPLC-TOF-MS

Pazopanib

Plasma

HPLC-MS/MS

Pemetrexed (Alimta®)

Plasma; IP fluid

HPLC-MS/MS

Pentosidine

Plasma

HPLC-FLD

PGE-M

Urine

HPLC-MS/MS

Phenethylamine

Urine

HPLC-MS/MS

Phthalates (mono- and di-butyl phthalate)

Plasma; Tissue

HPLC-MS/MS

PHT-427 (Akt/phosphatidylinositide-dependent
Protein Kinase 1 Pleckstrin Homology Domain Inhibitor)

Plasma; Tissue

HPLC-UV;
HPLC-MS/MS

PhytoCeramide

Yeast

*HPLC-MS/MS 

PMIP (therapeutic peptide)

Plasma

HPLC-MS/MS 

Polyamines

Plasma; Tissue

HPLC-FLD 

Psorospermin methyl ether

Plasma

HPLC-UV 

PX-12(1-methylpropyl 2-imidazolyl disulfide)

Plasma 

HPLC-UV;
HPLC-MS/MS

Quercetin

Plasma; skin

HPLC-MS/MS

Radicicol

Plasma

HPLC-MS/MS

Rapamycin

Whole blood

HPLC-MS/MS

Resveratrol

Plasma; Tissue

HPLC-MS/MS 

Retinols(Retinol, all-trans retinoic acid, 9-cis retinoic acid)

Plasma; Skin, Mouse Tissue

HPLC-FLD/HPLC-MS/MS

Selenium

Plasma, prostate tissue 

AA 

Serotonin

Plasma; Tissue; Urine

HPLC-MS/MS

SGM45

Mouse Plasma

HPLC-MS/MS

Sphingosine/Sphinogsine-1-P

Cells; plasma; Urine; tissue

*HPLC-MS/MS 

Sphingomyelin

Cells; plasma; Urine; tissue

*HPLC-MS/MS 

Sulindac/Sulindac metabolites

Plasma; Breast Fluid; Skin 

HPLC-MS/MS 

Targretin

Plasma

HPLC-FLD 

Taurine

Urine

HPLC-MS/MS

Tea Catechins

Plasma; Urine 

HPLC-EC 

Testosterone

Plasma

HPLC-MS/MS 

TC1S2(iron chelator)

Mouse Plasma

HPLC-MS/MS

TC4G6(iron chelator)

Mouse Plasma

HPLC-MS/MS

Thyroxine/Triiodothyroxine

Plasma 

HPLC-MS/MS 

Tocopherol/Tocopherol succinate

Plasma; Tissue 

HPLC-FLD

UA8967

Plasma

HPLC-MS/MS

Withaferin analogues

Plasma

HPLC-MS/MS 

Wortmannin analogues

Plasma; Bile

HPLC-MS/MS

Zileuton

Plasma

HPLC-MS/MS

 

*= available through Sub-core

**= 9-HOTrE, 13-HOTrE, 13-oxo-ODE, 9-oxo-ODE, 13-HODE, 9-HODE, 12(13)-EpOME, 9(10)-EpOME, EKODE, 12,13-DiHOME, 9,10-DiHOME, 15-deoxy-Prostaglandin J2, 15-HEPE, 8-HEPE, 12-HEPE, 5-HEPE, 17(18)-EpETE, 15-oxo-ETE, 14(15)-EpETE, 11(12)-EpETE, 8(9)-EpETE, 12-oxo-ETE, 5-oxo-ETE, 20-HETE, 15-HETE, 11-HETE, 8-HETE, 12-HETE, 9-HETE, 5-HETE, 14(15)-EET, 11(12)-EET, 8(9)-EET, 5(6)-EET, 15(S)-HETrE, Prostaglandin J2, Prostaglandin B2, 8,15-DiHETE, 6-trans-Leukotriene B4, 5,15-DiHETE, 17,18-DiHET, Leukotriene B4, 14,15-DiHETE, 5,6-DiHETE, 14,15-DiHET, Leukotriene B3, 11,12-DiHET, 8,9-DiHET, 5,6-DiHET, 17-HDHA, 19(20)-EpDPA, 16(17)-EpDPA, 13(14)-EpDPA, 10(11)-EpDPA, 7(8)-EpDPA, Prostaglandin E3, Prostaglandin D3, 20-OH-Leukotriene B4, Prostaglandin E2, Prostaglandin D2, Lipoxin A4, Prostaglandin F2a, Prostaglandin E1, Prostaglandin D1, 19,20-DiHDPE, 20-COOH-LTB4, 6-keto-PGF1a, TXB2, Resolvin D1

 

***= 3-hydroxyanthranilic acid, quinolinic acid, anthranilic acid, L-tryptophan, L-kynurenine, kynurenic acid, 3-hydroxykynurenine, cinnabarinic acid, xanthurenic acid, N-formylkynurenine, picolinic acid, 5-hydroxytryptophan, serotonin, melatonin, tryptamine, glutamic acid, dopamine

University of Arizona Cancer Center, Room 4965

Director
H-H. Sherry Chow, PhD
(520) 626-3358

Manager
Wade Chew
(520) 626-2331

Contact
UACC-ACSR@uacc.arizona.edu

Potential users, especially first-time users, are encouraged to personally contact ACSR service personnel for instructions on how to access all the services of the Analytical Chemistry Shared Resource. Please contact Dr. Chow by phone or email. After a short discussion about your project, the service request form can be submitted to the ACSR and a realistic time line can be projected.

All University of Arizona Cancer Center members have access to the Analytical Chemistry Shared Resource. The service is provided according to the following University of Arizona Cancer Center priority guidelines

  1. University of Arizona Cancer Center members with peer-reviewed extramural funding;
  2. New University of Arizona Cancer Center investigators with University of Arizona Cancer Center pilot project or developmental funding;
  3. University of Arizona Cancer Center members with other funding sources;
  4. Peer-reviewed, funded non-members at University of Arizona if sufficient time/resources are available to accommodate requested service.

 

Cancer Prevention and Control Program co-Leader
Shared Resource Director, Analytical Chemistry