Equipment

• The FACS Canto II (BD Biosciences, San Jose, CA) 
  • is an analyzer which can acquire data from eight fluorescence detectors using three lasers: 405nm, 488nm, and 640nm.
• The FACS Aria III (BD Biosciences, San Jose, CA)
  •  is a cell sorter equipped with 5 lasers: 488nm, 633nm, 561nm, 405nm and 355nm, and offers 17 fluorescence detectors. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture. The addition of a Baker BioProtect III biosafety cabinet, in which the Aria is housed, allows for sorting of certain live human tissue and BSL2 agents.
• The LSRII (BD Biosciences, San Jose, CA) 
  • is an analyzer capable of acquiring and analyzing data from 16 fluorescence parameters and has five lasers: 355nm, 405nm, 488nm, 532 nm and 640nm.
• The Cytek Aurora (Cytek Biosciences, Fremont, CA) 
  • is a multispectral flow cytometer. This instrument is capable of 64 channels of detection across 5 lasers using up to 40 colors in a single multicolor sample. Laser wavelengths include 355nm, 405nm, 488nm, 561nm, and 640nm. This cytometer also comes equipped with a 96 well plate loader for high throughput cytometry applications.
• The ImagestreamX MarkII 
  • is an imaging cytometer, which uses flow cytometry technology to acquire microscopic images of fluorescence data from thousands of individual cells in liquid suspension. It has 10 fluorescence channels, using four lasers: 405nm, 488nm, 532nm, and 640nm.
• The Fortessa (BD Biosciences, San Jose, CA) 
  • which has been in the care of the Nikolich-Zugich laboratory for many years is now administered by the FCIMSR. It can be found in the Medical Research Building, room 240K2, directly across from the LSRII flow cytometer. The Fortessa is functionally identical to the LSRII, but newer, with 7 detectors off the violet laser compared to only 4 detectors on the LSR.
• The Attune (ThermoFisher) 
  • is a 14 ‘color’ flow cytometer with an exceptionally fast tube and plate sample rate. It uses sound waves to focus the sample stream into the laser intercepts, so its data precision is almost unaffected by flow rate, unlike a conventional flow cytometer which uses fluid dynamics to focus the sample which cannot maintain precision at even moderate flow rates.
• The Seahorse XFe96 Metabolic Analyzer (Agilent Technologies, Santa Clara, CA) 
  • is used to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples
• The QuantStudio 3D Digital PCR System (Applied Biosystems, Foster City, CA)
  •  is a simple, affordable platform that uses sealed-chip technology, providing a streamlined, reliable, and robust method for performing digital PCR. The turn-key chip-based workflow allows for loading the chip, sealing and proceeding to amplification. Featuring 20,000 nanoscale reaction wells per chip, this platform enables more controlled sample loading and less reaction dropout than droplet-based approaches, ensuring higher precision for absolute quantification
• The BioTek Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments Winooski, VT) 
  • combines high sensitivity filter-based fluorescence and high contrast brightfield imaging with conventional multimode detection. The Cytation 1 can interface with the Seahorse instrument to allow for real-time normalization for Seahorse assays.
• The NanoString GeoMx Digital Spatial Profiler (DSP) (Nanostring Seattle, WA) 
  • is a spatial biology instrument that enables morphology-driven, high-plex profiling on a single FFPE or fresh frozen tissue section. DSP technology combines standard immunofluorescence techniques with nCounter optical barcoding quantification. This technology allows for spatially resolved, high-plex (10s -10,000s) digital quantitation of proteins or mRNA while maintaining integral information about tissue architecture and spatial relationships between cellular networks
• The Nanodrop One (ThermoScientific) 
  • is a compact, stand-alone UV-Visible spectrophotometer developed for micro-volume analysis of purified nucleic acids and a wide variety of proteins. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilution. The Nanodrop One comes with preloaded software and a touchscreen display.