Flow Cytometry & Human Immune Monitoring

Flow Cytometry

The Flow Cytometry Shared Resource (FCSR) at the University of Arizona Cancer Center supports the research needs of all Cancer Center members by providing state-of-the-art instrumentation for data acquisition, analysis, and cell sorting, and the technical expertise to interpret results, and develop methods. We offer information about new techniques, and applications of flow cytometry through workshops and seminars, and provide training to interested facility users who wish to run their own samples. Individual consultation services are available to discuss the specifics of each project.

Flow cytometry is a powerful tool that measures the functional and structural characteristics of heterogeneous mixtures of cells and particles in suspension based on their ability to scatter light. The cell sorting function separates these cells physically into their different classes. Researchers have the capability to analyze and sort cells by differences in physiology, metabolism, morphology and other characteristics. The ability to distinguish different cell types is limited only by the ability to attach specific fluorescent markers to the cells.

Human Immune Monitoring

The Human Immune Monitoring Facility (HIMF) is a shared resource within the UArizona Cancer Center that provides comprehensive immune profiling, sample preparation, and clinical trial support. Our mission is to provide technical expertise to clinical investigators and research scientists who want to characterize the immune system by offering up-to-date immune monitoring services for clinical and translational studies.

Our services include multispectral flow cytometry, metabolic analysis, blood services such as PBMC isolation and serum/plasma extraction, and a full spectrum of immunological services. We also offer custom assays upon request and will work with interested investigators to help get their projects up and running. Biobanking services are also available upon request.

We offer training, assisted, unassisted, and technician operated modalities for all our instruments and will meet investigators at their desired level of engagement. All services are available during regular business hours (M-F 8am - 4pm) with 24 hour access to select instruments arriving in the near future.

To build a project, get a quote, or order a service within iLab:
Step 1: Select “About Our Core” to learn more about this Shared Resource.
Step 2: Click here to login or sign up for an iLab account at the University of Arizona. For login help, email iLab-support@agilent.com.
Step 3: Once the login step is completed, obtaining prices, services and scheduling is available within the iLab system.

Please remember to acknowledge the Cancer Center Support Grant (P30 CA023074) when publishing manuscripts or abstracts that utilized the services of the University of Arizona Cancer Center’s Shared Resources and/or were derived from CCSG pilot funds. Suggested language: "Research reported in this [publication/press release] was supported by the National Cancer Institute of the National Institutes of Health under award number P30 CA023074.

Equipment

  • The FACScanto II (BD Biosciences, San Jose, CA) is an analyzer, which can acquire data from eight fluorescence detectors using three lasers: 405nm, 488nm, and 640nm.
  • The FACSAria III (BD Biosciences, San Jose, CA) is a cell sorter equipped with 5 lasers: 488nm, 633nm, 561nm, 405nm and 355nm, and offers 17 fluorescence detectors. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture. The addition of a Baker BioProtect III biosafety cabinet, in which the Aria is housed, allows for sorting of certain live human tissue and BSL2 agents.
  • The LSRII (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from 16 fluorescence parameters and has five lasers: 355nm, 405nm, 488nm, 532 nm and 640nm.
  • The Cytek Aurora (Cytek Biosciences, Fremont, CA) is a multispectral flow cytometer. This instrument is capable of 64 channels of detection across 5 lasers using up to 40 colors in a single multicolor sample. Laser wavelengths include 355nm, 405nm, 488nm, 561nm, and 640nm. This cytometer also comes equipped with a 96 well plate loader for high throughput cytometry applications.
  • The ImagestreamX markII is an imaging cytometer, which uses flow cytometry technology to acquire microscopic images of fluorescence data from thousands of individual cells in liquid suspension. It has 10 fluorescence channels, using four lasers: 405nm, 488nm, 532nm, and 640nm.
  • The Seahorse XFe96 Metabolic Analyzer (Agilent Technologies, Santa Clara, CA) is used to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples
  • The QuantStudio 3D Digital PCR System (Applied Biosystems, Foster City, CA) is a simple, affordable platform that uses a sealed-chip technology, providing a streamlined, reliable, and robust method for performing digital PCR. The turn-key chip-based workflow lets you load the chip, seal and proceed to amplification. Featuring 20,000 nanoscale reaction wells per chip this platform enables more controlled sample loading and less reaction dropout than droplet-based approaches, ensuring higher precision for absolute quantification

     

 

Flow Cytometry – University of Arizona Cancer Center, Room 0935

Human Immune Monitoring – University of Arizona Cancer Center, Room 0913

Director
Sara Centuori, PhD
520-626-3249

Manager
Mark Curry
979-739-5709

Contact
UACC-FCSR@uacc.arizona.edu

External Website

ARL/University of Arizona Cancer Center Cytometry Core Facility

Shared Resource Director, Flow Cytometry