The mission of the Proteomics Shared Resource (PSR) is to provide the University of Arizona Cancer Center investigators with a dedicated facility and expertise in analyzing proteins for their identity, quantity and function via state-of-the art Modern Mass Spectrometry and peripheral analytical instrumentation.
Over the past funding cycle, the PSR has performed proteomics analyses for Cancer Center members belonging to the four scientific research programs.
Please remember to acknowledge the Cancer Center Support Grant (P30 CA023074) when publishing manuscripts or abstracts that utilized the services of the University of Arizona Cancer Center’s Shared Resources and/or were derived from CCSG pilot funds. Suggested language: "Research reported in this [publication/press release] was supported by the National Cancer Institute of the National Institutes of Health under award number P30 CA023074.
At the Proteomics Core Facility (Analytical & Biological Mass Spectrometry Core Facility), we have state-of-the-art equipment and unparalleled expertise for the analysis for a variety of bio and small molecular analyses. One of our specializations include protein-analysis services encompassing protein identification, protein and peptide sequence confirmation, intact protein molecular weight determination, and complex sample analysis. We can also provide information about protein/antibody-drug interactions.
We also study a range of post-translational modification (PTM) identification and quantitation. These services include analyzing a sample for the presence or absence of a particular PTM on a target or untargeted protein sample.
Our services are designed to assist you to answer your quantitative proteomics and small molecule questions as well. The services include workflows for label free, chemical labeling (iTRAQ/TMT) and metabolic labeling (SILAC).
For chemists who would like to synthesize a molecule, we monitor the reaction step-by-step, calculate the percentage of product, test for its purity, and characterize the molecule with high resolution and high mass accuracy (typically better than 500 ppb [0.5 ppm] mass accuracy).
Whether you are interested in molecular weight and chemical composition determinations, structure elucidations and compound identification analysis or confirmation and accurate mass measurements of synthetic products, measurement of polymers, nucleic acids (DNA/RNA), peptides, proteins, natural products, and assistance with determination of unknowns or characterization of biological proteins, we look forward to supporting your research needs.
- Gel electrophoresis (achieves separation of proteins in a gel matrix)
- Protein assay
- Intact protein analysis
- Sample cleanup (zip tip, protein precipitation, SPE)
- Low Resolution and High Resolution MS of small molecules (IT MS, FT-MS or MALDI-TOF MS)
- Low Resolution MS separation (GC-MS or LC-MS)
- HPLC-MS and HPLC-MS/MS method development
- Orbitrap MS or FTICR MS (high resolution MS)
- Orbitrap LC-MS/MS of a protein(s) digest from solution/gel band (staff or self-digested)
- Chromatography small molecule purification/fractionation
- LC-MS and LC-MS/MS
- Abundant protein depletion
- Chromatography protein/peptide purification
- Sample concentration and clean-up
- Aid with experimental design and data/results interpretation
- Q Exactive Plus (Thermo); 2016 University of Arizona purchase; Dionex Nano- HPLC, nanoESI source (Thermo), HCD, Deep and Greater Proteomic Profiling
- Q Exactive Plus (Thermo); 2016 University of Arizona purchase; Dionex Vantage HPLC, ESI source (Thermo), HCD, Lipid analysis (in development)
- LTQ Orbitrap Velos (Thermo); 2009 NIH NCRR HEI; Proxeon Nano- HPLC, ESI source (Advion), CID, ETD; Proteomic Profiling, PTM identification
- 9.4t solariX 2XR FTMS (Bruker); 2018 University of Arizona purchase HEI; ESI, nano-ESI, MALDI; CID, ECD, ETD, SORI, IRMPD; high-resolution MS
- AmaZon 3D Ion Trap (Bruker); 2016 University of Arizona purchase; small molecule rapid MS analysis
- Autoflex Speed MALDI-TOF (Bruker); 2016 University of Arizona purchase; high throughput MS measurements of polymers, and whole proteins
- Gel separation and analyses: 1D and 2D gel stations.
- Off-line LC separation: AKTA FPLC; Michrom Paradigm LC
- Orbitrap Exploris 480 (Thermo couple with Vanquish Duo HPLC and UHPLC System: 2020 University of Arizona purchase; Next Generation advanced high resolution accurate mass Quadrupole-Orbitrap MS – high performance and throughput for metabolite and lipid analysis.
Room 124J, BIO5 Keating Bioresearch Building, 1657 E. Helen St.
Email us with inquiries today at MassSpec@email.arizona.edu.
Krishna Parsawar, PhD
Cynthia David, PhD
Assistant Staff Scientist
Tel: (520) 626-4173
Yelena Feinstein, MS
Tel: (520) 626-4161
Kristen Kallen-Keck, BS
Analytical Research Coordinator